Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Claudin 5 (Phospho-Tyr217) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | Claudin 5 (phospho Tyr217) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLD5, CLDN5, TMDVCF |
| Product description | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome.1) Sirotkin H., Genomics 42:245-251(1997). |
| Immunogen | Claudin 5 (phospho Tyr217) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 217 (K-K-N-Y (p)-V) derived from Human Claudin 5. |
| Modification | p-Tyr217 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN5 |
|---|---|
| Alternative Names | CLD5 CLDN5 TMDVCF |
| Molecular Weight(MW) | 23 kDa |
| Tissue Specificity | The antibody detects endogenous levels of Claudin 5 only when phosphorylated at tyrosine 217. |
| Entrez Gene | 7122 |
|---|---|
| Protein Accession | NP_001124333.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using Claudin 5 (Phospho-Tyr217) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
