The CLCN family of voltage-dependent chloride channel genes comprises nine members (CLCN1-7, Ka and Kb) which demonstrate quite diverse functional characteristics while sharing significant sequence homology. Chloride channel 6 and 7 belong to a subbranch of this family. Chloride channel 6 has four different alternatively spliced transcript variants. This gene is in close vicinity to two other kidney-specific chloride channel genes, CLCNKA and CLCNKB.1) Tran, P., et al., (2002) Mamm. Genome 13 (9), 483-492.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLCN6.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CLCN6 antibody can be used for detection of CLCN6 by ELISA at 1:62500. CLCN6 antibody can be used for detection of CLCN6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human NT-2, Mouse WT brain at 2 ug/ml (Lane 1. Human NT-2 cells (60ug) lysate 2. Mouse WT brain extract (80ug)).
Application
Antibody used in WB on Human Jurkat 1.25 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
CLCN6 antibody can be used for detection of CLCN6 by ELISA at 1:62500. CLCN6 antibody can be used for detection of CLCN6 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLCN6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt