Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Product Name | CLDN22 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Claudin-22, CLDN22 |
| Product description | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is intronless and overlaps the 3' UTR of the WWC2 gene (GeneID: 80014) on the opposite strand.1) Lal-Nag, M., et al. Genome Biol. 10 (8), 235 (2009) : |
| Immunogen | This CLDN22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-117 amino acids from the Central region of human CLDN22. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN22 |
|---|---|
| Alternative Names | Claudin-22 CLDN22 |
| Molecular Weight(MW) | 25 kDa |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
