CLDN8, clustered with CLDN17 at human chromosome 21q22.11, is a four-transmembrane protein with WWCC motif, defined by W-X (17-22)-W-X (2)-C-X (8-10)-C.1) Douglas A. Melton, et al., (2007) Dev Biol 304:541.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CLDN8.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
CLDN8 antibody can be used for detection of CLDN8 by ELISA at 1:62500. CLDN8 antibody can be used for detection of CLDN8 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.5-1.0 ug/ml.
Application
Antibody used in IHC on Human Intestine.
Application
Antibody used in IHC on Human kidney.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
CLDN8 antibody can be used for detection of CLDN8 by ELISA at 1:62500. CLDN8 antibody can be used for detection of CLDN8 by western blot at 0.5-1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CLDN8 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt