Application
Immunofluorescence of CLEC2A in K562 cells with CLEC2A antibody at 20 μg/mL.Application
Western blot analysis of CLEC2A in K562 cell lysate with CLEC2A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Product Name | CLEC2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLEC2A Antibody: KACL, PILAR, UNQ5792, KACL, UNQ5792/PRO19597, C-type lectin domain family 2 member A, Keratinocyte-associated C-type lectin |
| Product description | CLEC2A Antibody: CLEC2A (C-type lectin domain family 2 member A), also known as PILAR (proliferation-induced lymphocyte-associated receptor) is involved in modulating T-cell expansion. It is a 32 kDa single-pass type II membrane protein that contains one C-type lectin domain in its extracellular region and belongs to the CTL/CTLD superfamily. CLEC2A is mainly expressed in skin and highly expressed in CD8(+), B lymphocytes and naive CD4(+) T cells. Manipulation of CLEC2A signaling may be important for treatment of autoimmune diseases and cancer.1) Huarte E, Cubillos-Ruiz JR, Nesbeth YC, et al. PILAR is a novel modulator of human T-cell expansion. Blood 2008; 112:1259-68. |
| Immunogen | CLEC2A antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CLEC2A. The immunogen is located within the first 50 amino acids of CLEC2A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CLEC2A antibody can be used for detection of CLEC2A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLEC2A |
|---|---|
| Alternative Names | CLEC2A Antibody: KACL PILAR UNQ5792 KACL UNQ5792/PRO19597 C-type lectin domain family 2 member A Keratinocyte-associated C-type lectin |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | CLEC2A antibody is human specific. Two alternatively spliced transcript variants have been observed. |
| Entrez Gene | 387836 |
|---|---|
| Protein Accession | NP_001124183 |
Application
Immunofluorescence of CLEC2A in K562 cells with CLEC2A antibody at 20 μg/mL.
Application
Western blot analysis of CLEC2A in K562 cell lysate with CLEC2A antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | CLEC2A antibody can be used for detection of CLEC2A by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CLEC2A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CLEC2A Antibody is supplied in PBS containing 0.02% sodium azide. |
