Application
Immunohistochemistry of CLEC7A in human spleen tissue with CLEC7A antibody at 5 μg/mL.Application
Western blot analysis of CLEC7A in rat spleen tissue lysate with CLEC7A antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of CLEC7A in human spleen tissue withCLEC7A antibody at 20 μg/mL.| Product Name | CLEC7A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLEC7A Antibody: BGR, CANDF4, DECTIN1, CLECSF12, BGR, UNQ539/PRO1082, C-type lectin domain family 7 member A, Beta-glucan receptor, DC-associated C-type lectin 1 |
| Product description | CLEC7A Antibody: CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.1) Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J. Immunol. 2002; 169:3876-82. |
| Immunogen | CLEC7A antibody was raised against a 17 amino acid synthetic peptide near the center of human CLEC7A. The immunogen is located within amino acids 70 - 120 of CLEC7A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CLEC7A antibody can be used for detection of CLEC7A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLEC7A |
|---|---|
| Alternative Names | CLEC7A Antibody: BGR CANDF4 DECTIN1 CLECSF12 BGR UNQ539/PRO1082 C-type lectin domain family 7 member A Beta-glucan receptor DC-associated C-type lectin 1 |
| Molecular Weight(MW) | Predicted: 27 kDa Observed: 27 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | Multiple isoforms of CLEC7A are known to exist. |
Application
Immunohistochemistry of CLEC7A in human spleen tissue with CLEC7A antibody at 5 μg/mL.
Application
Western blot analysis of CLEC7A in rat spleen tissue lysate with CLEC7A antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of CLEC7A in human spleen tissue withCLEC7A antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | CLEC7A antibody can be used for detection of CLEC7A by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CLEC7A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CLEC7A Antibody is supplied in PBS containing 0.02% sodium azide. |
