WB
Western blot analysis of CLEC7A using anti-CLEC7A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CLEC7A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of CLEC7A using anti-CLEC7A antibody. CLEC7A was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-CLEC7A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.FC
Flow Cytometry analysis of ANA-1 cells using anti-CLEC7A antibody. Overlay histogram showing ANA-1 cells stained with anti-CLEC7A antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CLEC7A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Anti-CLEC7A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived mouse Dectin-1/Clec7a recombinant protein (Position: Q17-L244). Mouse Clec7a shares 95.2% amino acid (aa) sequence identity with human Clec7a. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-2000 IHC:1:50-400 FC:1:50-200 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLEC7A |
|---|---|
| Gene Synonyms | BGR CD369 CANDF4 SCARE2 DECTIN1 CLECSF12 |
| Gene Full Name | C-type lectin domain containing 7A |
| Gene Summary | This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. The encoded glycoprotein is a small type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif. It functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008] |
| Alternative Names | CLEC7A BGR CLECSF12 DECTIN1 UNQ539/PRO1082 C-type lectin domain family 7 member A Beta-glucan receptor C-type lectin superfamily member 12 Dendritic cell-associated C-type lectin 1 DC-associated C-type lectin 1 Dectin-1 CD369 CANDF4 SCARE2 C-type lectin domain containing 7A |
| Molecular Weight(MW) | 27kDa(Observed MW 36kDa) |
| Cellular Localization | Cell membrane |
WB
Western blot analysis of CLEC7A using anti-CLEC7A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates, Lane 2: mouse thymus tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CLEC7A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of CLEC7A using anti-CLEC7A antibody. CLEC7A was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-CLEC7A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
FC
Flow Cytometry analysis of ANA-1 cells using anti-CLEC7A antibody. Overlay histogram showing ANA-1 cells stained with anti-CLEC7A antibody (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CLEC7A Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-2000 IHC:1:50-400 FC:1:50-200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
