Application
Immunofuorescence of CLIP170 in human brain tissue with CLIP170 antibody at 20 μg/mL.Application
Western blot analysis of CLIP170 in rat brain tissue lysate with CLIP170 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | CLIP170 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLIP170 Antibody: RSN, CLIP, CYLN1, CLIP170, CLIP-170, RSN, CAP-Gly domain-containing linker protein 1, Cytoplasmic linker protein 1 |
| Product description | CLIP170 Antibody: CLIP170 was initially identified as a new type of intermediate filament associated protein that is highly expressed in Reed-Sternberg cells, the tumoral cells diagnostic for Hodgkin's disease. Later experiments showed that it is located at microtubule plus ends and is required for the binding of endocytic carrier vesicles. CLIP170 has also been suggested to act with LIS1, a protein implicated in brain development, to regulate dynein/dynactin binding microtubules. Other studies suggest that CLIP170 can influence the formation of lamellipodia and cell invasion by invasive breast cancer cells by regulating the release of kinesin and IQGAP1 from a complex of those proteins, CLIP170 and Rac1. At least two isoforms of CLIP170 are known to exist.1) Bilbe G, Delabie J, Bruggen J, et al. Restin: a novel intermediate filament-associated protein highly expressed in the Reed-Sternberg cells of Hodgkin’s disease. EMBO J.1992; 11:2103-13. |
| Immunogen | CLIP170 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human CLIP170. The immunogen is located within amino acids 1340 - 1390 of CLIP170. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CLIP170 antibody can be used for detection of CLIP170 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLIP1 |
|---|---|
| Alternative Names | CLIP170 Antibody: RSN CLIP CYLN1 CLIP170 CLIP-170 RSN CAP-Gly domain-containing linker protein 1 Cytoplasmic linker protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofuorescence of CLIP170 in human brain tissue with CLIP170 antibody at 20 μg/mL.
Application
Western blot analysis of CLIP170 in rat brain tissue lysate with CLIP170 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | CLIP170 antibody can be used for detection of CLIP170 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CLIP170 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CLIP170 Antibody is supplied in PBS containing 0.02% sodium azide. |
