Application
Flow cytometric analysis of hela cells using CLNS1A Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of CLNS1A antibody in HL60 cell line lysates (35ug/lane)Application
Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | CLNS1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Methylosome subunit pICln, Chloride channel, nucleotide sensitive 1A, Chloride conductance regulatory protein ICln, I(Cln), Chloride ion current inducer protein, ClCI, Reticulocyte pICln, CLNS1A, CLCI, ICLN |
| Product description | CLNS1A is a protein that functions in multiple regulatory pathways. The protein complexes with numerous cytosolic proteins and performs diverse functions including regulation of small nuclear ribonucleoprotein biosynthesis, platelet activation and cytoskeletal organization. The protein is also found associated with the plasma membrane where it functions as a chloride current regulator.1) Yeung,C.H., Biol. Reprod. 73 (5), 1057-1063 (2005) |
| Immunogen | This CLNS1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-216 amino acids from the C-terminal region of human CLNS1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CLNS1A |
|---|---|
| Alternative Names | Methylosome subunit pICln Chloride channel nucleotide sensitive 1A Chloride conductance regulatory protein ICln I(Cln) Chloride ion current inducer protein ClCI Reticulocyte pICln CLNS1A CLCI ICLN |
| Molecular Weight(MW) | 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rb |
Application
Flow cytometric analysis of hela cells using CLNS1A Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CLNS1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of CLNS1A antibody in HL60 cell line lysates (35ug/lane)
Application
Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
