Application
Immunohistochemistry of CLPH in human testis tissue with CLPH antibody at 5 μg/mL.Application
Western blot analysis of CLPH in human testis tissue lysate with CLPH antibody at 1 μg/mL.| Product Name | CLPH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLPH Antibody: CLPH, C4orf35, NYD-SP26, Calcium-binding and spermatid-specific protein 1, Testis development protein NYD-SP26 |
| Product description | CLPH Antibody: CLPH (Casein-Like phosphoprotein) is a recently discovered, evolutionarily conserved, testis-specific cytoplasmic protein expressed exclusively in postmeiotic germ cells in rat spermatogenesis. CLPH is a novel casein kinase 2-phosphorylated disordered and calcium-binding protein that displays strong variation in relative expression levels during spermatogenesis. It is frequently associated with the mitochondrial inner membrane during the last steps of spermatid differentiation and is involved in the extremely complex structural rearrangements occurring in haploid germ cells during spermiogenesis.1) Calvel P, Kervarrec C, Lavigne R, et al. CLPH, a novel casein kinase 2-phosphorylated disordered protein, is specifically associated with postmeiotic germ cells in rat spermatogenesis. J. Proteome Res.2009; 8:2953-65. |
| Immunogen | CLPH antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human CLPH. The immunogen is located within the last 50 amino acids of CLPH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| CLPH antibody can be used for detection of CLPH by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C4orf35 |
|---|---|
| Alternative Names | CLPH Antibody: CLPH C4orf35 NYD-SP26 Calcium-binding and spermatid-specific protein 1 Testis development protein NYD-SP26 |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
Application
Immunohistochemistry of CLPH in human testis tissue with CLPH antibody at 5 μg/mL.
Application
Western blot analysis of CLPH in human testis tissue lysate with CLPH antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | CLPH antibody can be used for detection of CLPH by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CLPH antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CLPH Antibody is supplied in PBS containing 0.02% sodium azide. |
