Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CLTA using rabbit polyclonal CLTA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTA gene.Application
Western blot analysis of CLTA Antibody in MDA-MB231 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CLTA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CLTA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Clathrin light chain A, Lca, CLTA |
| Product description | Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. CLTA is one of two clathrin light chain proteins which are believed to function as regulatory elements.1) Wong,D.H., et.al., J. Neurosci. 10 (9), 3025-3031 (1990) Nathke,I., et.al., J. Biol. Chem. 265 (30), 18621-18627 (1990) |
| Immunogen | This CLTA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-127 amino acids from the Central region of human CLTA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLTA |
|---|---|
| Alternative Names | Clathrin light chain A Lca CLTA |
| Molecular Weight(MW) | 27 kDa |
Application
Flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CLTA using rabbit polyclonal CLTA Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLTA gene.
Application
Western blot analysis of CLTA Antibody in MDA-MB231 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CLTA Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
