Application
Flow cytometric analysis of CEM cells using CNOT8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in U251 cell line lysates (35ug/lane).Application
Fluorescent confocal image of Hela cell stained with CNOT8 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidi| Product Name | CNOT8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCR4-NOT transcription complex subunit 8, CAF1-like protein, CALIFp, CAF2, CCR4-associated factor 8, Caf1b, CNOT8, CALIF, POP2 |
| Product description | Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex.1) Morel,A.P., J. Cell. Sci. 116 (PT 14), 2929-2936 (2003) |
| Immunogen | This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CNOT8 |
|---|---|
| Alternative Names | CCR4-NOT transcription complex subunit 8 CAF1-like protein CALIFp CAF2 CCR4-associated factor 8 Caf1b CNOT8 CALIF POP2 |
| Molecular Weight(MW) | 34 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of CEM cells using CNOT8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in U251 cell line lysates (35ug/lane).
Application
Fluorescent confocal image of Hela cell stained with CNOT8 Antibody .Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidi| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
