Application
This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.| Product Name | CNTF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ciliary neurotrophic factorCNTF |
| Product description | Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant rCNTF (Rat Ciliary Neurotrophic Factor). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISANeutWB |
| Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/mL), a concentration of 0.02 - 0.05 μg/mL of this antibody is required. ELISA: To detect rCNTF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect rCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cntf |
|---|---|
| Alternative Names | Ciliary neurotrophic factorCNTF |
Application
This antibody stained colchicine injected rat brain (including the globus pallidus region) tissue. The primary antibody was incubated at 0.25 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary.
Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Rat CNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF.| Application Notes | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of rCNTF (0.15 ng/mL), a concentration of 0.02 - 0.05 μg/mL of this antibody is required. ELISA: To detect rCNTF by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rCNTF. Sandwich: To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Rat CNTF (XP-5114Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect rCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CNTF antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
