Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using coflin1/cofilin2 (Ab-88). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using coflin1/cofilin2 (Ab-88).Application
Western blot analysis of lysed extracts from MDA cells using coflin1/cofilin2 (Ab-88).| Product Name | coflin1 / cofilin2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CFL, HEL-S-15, RNF53, Cofilin, non-muscle isoform 18 kDa phosphoprotein |
| Product description | Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.1) Carlier, M. et al. (1999) J. Biol. Chem. 274, 33827-33830. Arber, S. et al. (1998) Nature 393, 805-809. Yang, N. et al. (1998) Nature 393, 809-812. |
| Immunogen | coflin1/cofilin2 (Ab-88) antibody was raised against a peptide sequence around aa. 86~90 (A-T-Y-E-T) derived from Human coflin1/cofilin2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CFL1 CFL2 |
|---|---|
| Alternative Names | CFL HEL-S-15 RNF53 Cofilin non-muscle isoform 18 kDa phosphoprotein |
| Molecular Weight(MW) | 19 kDa |
| Tissue Specificity | This antibody detects endogenous level of total cofilin1/cofilin2 protein. |
| Entrez Gene | 1072 / 1073 |
|---|---|
| Protein Accession | NP_005498.1 / NP_068733.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using coflin1/cofilin2 (Ab-88). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using coflin1/cofilin2 (Ab-88).
Application
Western blot analysis of lysed extracts from MDA cells using coflin1/cofilin2 (Ab-88).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
