Application
Immunofluorescence of COH1 in human brain tissue with COH1 antibody at 20 μg/mL.Application
Western blot analysis of COH1 in SK-N-SH cell lysate with COH1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | COH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | COH1 Antibody: COH1 (Cohen syndrome protein 1), also known as VPS13B (vacuolar protein sorting-associated protein 13B) or CHS1, belongs to the VPS13 family and may function in vesicle-mediated transport and sorting of proteins within the cell. COH1 is widely expressed and multiple alternatively spliced transcript variants have been observed. Mutations in this gene have been associated with Cohen syndrome. COH1 is a Golgi-localized peripheral membrane protein and plays a critical role in Golgi (re)assembly.1) Velayos-Baeza A, Vettori A, Copley RR, et al. Analysis of the human VPS13 gene family. Genomics 2004; 84:536-49. |
| Immunogen | COH1 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human COH1. The immunogen is located within amino acids 90 - 140 of COH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISA,IF,WB |
| COH1 antibody can be used for detection of COH1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | VPS13B |
|---|---|
| Alternative Names | COH1 Antibody: CHS1 COH1 CHS1 KIAA0532 Vacuolar protein sorting-associated protein 13B Cohen syndrome protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least five alternatively spliced transcript variants have been observed. COH1 detects two isoforms. |
Application
Immunofluorescence of COH1 in human brain tissue with COH1 antibody at 20 μg/mL.
Application
Western blot analysis of COH1 in SK-N-SH cell lysate with COH1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | COH1 antibody can be used for detection of COH1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | COH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | COH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
