Application
Immunohistochemistry staining of Collagen III in testis (formalin-fixed paraffin embedded) using Collagen III Antibody.Application
Immunohistochemistry staining of Collagen III in kidney (formalin-fixed paraffin embedded) tissue using Collagen III Antibody.| Product Name | Collagen III Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EDS4A |
| Product description | Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens are extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used. |
| Immunogen | Collagen III antibody was raised against Full length Collagen Type III with intact 3-dimensional helical structure from human and bovine placenta. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| Collagen III antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | BovineHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COL3A1 |
|---|---|
| Alternative Names | EDS4A |
Application
Immunohistochemistry staining of Collagen III in testis (formalin-fixed paraffin embedded) using Collagen III Antibody.
Application
Immunohistochemistry staining of Collagen III in kidney (formalin-fixed paraffin embedded) tissue using Collagen III Antibody.| Application Notes | Collagen III antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store Collagen III antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.125 M sodium borate, 0.075 M sodium chloride, 0.005 M EDTA;, pH 8.0, 0.01% sodium azide. |
Data sheet for OM275892