Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: human placenta lysate Lysates/proteins at 20 ug per lane.| Product Name | COMMD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COMM domain-containing protein 1, Protein Murr1, COMMD1, C2orf5, MURR1 |
| Product description | Proposed scaffold protein that is implicated in diverse physiological processes and whose function may be in part linked to its ability to regulate ubiquitination of specific cellular proteins. Can modulate activity of cullin-RING E3 ubiquitin ligase (CRL) complexes by displacing CAND1; in vitro promotes CRL E3 activiy and dissocoiates CAND1 from CUL1 and CUL2 (PubMed: 21778237 ). Promotes ubiquitination of NF-kappa-B subunit RELA and its subsequent proteasomal degradation. Down-regulates NF-kappa-B activity (PubMed: 15799966 , PubMed: 17183367 , PubMed: 20048074 ). Involved in the regulation of membrane expression and ubiquitination of SLC12A2 (PubMed: 23515529 ). Modulates Na(+) transport in epithelial cells by regulation of apical cell surface expression of amiloride-sensitive sodium channel (ENaC) subunits and by promoting their ubiquitination presumably involving NEDD4L. Promotes the localization of SCNN1D to recycling endosomes (PubMed: 14645214 , PubMed: 20237237 , PubMed: 21741370 ). Promotes CFTR cell surface expression through regulation of its ubiquitination (PubMed: 21483833 ). Down-regulates SOD1 activity by interfering with its homodimerization (PubMed: 20595380 ). Plays a role in copper ion homeostasis. Involved in copper-dependent ATP7A trafficking between the trans- Golgi network and vesicles in the cell periphery; the function is proposed to depend on its association within the CCC complex and cooperation with the WASH complex on early endosomes (PubMed: 25355947 ). Can bind one copper ion per monomer (PubMed: 17309234 ). May function to facilitate biliary copper excretion within hepatocytes. Binds to phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2) (PubMed: 18940794 ). Involved in the regulation of HIF1A-mediated transcription; competes with ARNT/Hif-1-beta for binding to HIF1A resulting in decreased DNA binding and impaired transcriptional activation by HIF-1 (PubMed: 20458141 ).1) Mueller T.,et al.J. Hepatol. 38:164-168(2003). |
| Immunogen | This COMMD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 4--33 amino acids from human COMMD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COMMD1 |
|---|---|
| Alternative Names | COMM domain-containing protein 1 Protein Murr1 COMMD1 C2orf5 MURR1 |
| Molecular Weight(MW) | 21 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: U-2OS whole cell lysate Lane 5: Jurkat whole cell lysate Lane 6: human placenta lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
