Application
Western blot analysis of GJB4 using rabbit polyclonal GJB4 Antibody (H105.Connexin) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GJB4 gene.| Product Name | Connexin 30.3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gap junction beta-4 protein, Connexin-303, Cx303, GJB4 |
| Product description | Gap junctions permit direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular communication. Gap junction channels consist of connexin protein subunits encoded by a multigene family. Erythrokeratodermia variabilis (EKV) is an autosomal dominant disorder of keratinization characterized by migratory erythematous lesions and fixed keratotic plaques. Mutations in the GJB3 gene have been reported in some but not all families, although it has been postulated that the absence of connexin 30.3 can be compensated by other connexins.1) Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). |
| Immunogen | This Connexin 30.3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 90-120 amino acids from the N-terminal region of human Connexin 30.3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GJB4 |
|---|---|
| Alternative Names | Gap junction beta-4 protein Connexin-303 Cx303 GJB4 |
| Molecular Weight(MW) | 30 kDa |
Application
Western blot analysis of GJB4 using rabbit polyclonal GJB4 Antibody (H105.Connexin) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GJB4 gene.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
