Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | Connexin 36 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gap junction delta-2 protein, Connexin-36, Cx36, Gap junction alpha-9 protein, GJD2, GJA9 |
| Product description | GJA9, also called connexin-36 (CX36), is a member of the connexin gene family that is expressed predominantly in mammalian neurons. Connexins associate in groups of 6 and are organized radially around a central pore to form connexons. Each gap junction intercellular channel is formed by the conjunction of 2 connexons.1) de Brouwer, A.P., et al., Hum. Genet. 112(2):156-163 (2003). |
| Immunogen | This Connexin 36 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291-321 amino acids from the C-terminal region of human Connexin 36. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Symbol | GJD2 |
|---|---|
| Alternative Names | Gap junction delta-2 protein Connexin-36 Cx36 Gap junction alpha-9 protein GJD2 GJA9 |
| Molecular Weight(MW) | 36 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western Blot at 1:2000 dilution Lane 1: human brain lysate Lane 2: mouse brain lysate Lane 3: rat brain lysate Lane 4: SH-SY5Y whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
