Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-GJA8 Antibody in mouse kidney tissue lysates (35ug/lane)| Product Name | Connexin 50 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Gap junction alpha-8 protein, Connexin-50, Cx50, Lens fiber protein MP70, GJA8 |
| Product description | GJA8 is a an integral membrane protein that belongs to the connexin family, alpha-type (group II) subfamily. One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins. This particular connexin only forms junctional channels. GJA8 is expressed in the eye lens, and defects in GJA8 are the cause of zonular pulverulent cataract type 1 (CZP1), a form of autosomal dominant congenital cataract.1) Shiels, A., et al., Am. J. Hum. Genet. 62(3):526-532 (1998). Church, R.L., et al., Curr. Eye Res. 14(10):979-981 (1995). Church, R.L., et al., Curr. Eye Res. 14(3):215-221 (1995). |
| Immunogen | This Connexin 50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-433 amino acids from the C-terminal region of human Connexin 50. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | GJA8 |
|---|---|
| Alternative Names | Gap junction alpha-8 protein Connexin-50 Cx50 Lens fiber protein MP70 GJA8 |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-GJA8 Antibody in mouse kidney tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
