Application
Immunohistochemistry of Coronin 7 in rat lung tissue with Coronin 7 antibody at 2.5 μg/mL.Application
Immunofluorescence of Coronin 7 in rat lung tissue with Coronin 7 antibody at 20 μg/mL.Application
Western blot analysis of Coronin 7 in rat lung tissue lysate with Coronin 7 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Coronin 7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Coronin 7 Antibody: CRN7, POD1, 0610011B16Rik, Coronin-7, 70 kDa WD repeat tumor rejection antigen homolog, Crn7 |
| Product description | Coronin 7 Antibody: Coronins, such as Coronin 7, constitute an evolutionarily conserved family of WD-repeat actin-binding proteins whose function is thought to be regulating the cytoskeleton. Unlike other cytoplasmically localized Coronins, Coronin 7 localizes in part to the Golgi complex and is thought to play a role in Golgi complex morphology and function. Other experiments indicate that Coronin 7 interacts with the anti-proliferative protein TOB1 and enhances its degradation though the SCF ubiquitin ligase (E3) complex.1) Rybakin V, Stumpf N, Schulze A, et al. Coronin 7, the mammalian POD-1 homologue, localizes to the Golgi apparatus. FEBS Lett. 2004; 573:161-7. |
| Immunogen | Coronin 7 antibody was raised against an 17 amino acid synthetic peptide near the carboxy terminus of human Coronin 7. The immunogen is located within the last 50 amino acids of Coronin 7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Coronin 7 antibody can be used for detection of Coronin 7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CORO7 |
|---|---|
| Alternative Names | Coronin 7 Antibody: CRN7 POD1 0610011B16Rik Coronin-7 70 kDa WD repeat tumor rejection antigen homolog Crn7 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | Multiple isoforms of Coronin 7 are known to exist |
Application
Immunohistochemistry of Coronin 7 in rat lung tissue with Coronin 7 antibody at 2.5 μg/mL.
Application
Immunofluorescence of Coronin 7 in rat lung tissue with Coronin 7 antibody at 20 μg/mL.
Application
Western blot analysis of Coronin 7 in rat lung tissue lysate with Coronin 7 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1462 - Rat Lung Tissue Lysate |
|---|---|
| Application Notes | Coronin 7 antibody can be used for detection of Coronin 7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Coronin 7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Coronin 7 Antibody is supplied in PBS containing 0.02% sodium azide. |
