Application
Antibody used in WB on Human HepG2 cells at 5.0 ug/ml.Application
Antibody used in WB on Human fibroblast mitochondria at: 1:1000.Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.| Product Name | COX15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | COX15, CEMCOX2 |
| Product description | Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane.Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be essential for the biogenesis of COX formation and may function in the hydroxylation of heme O, according to the yeast mutant studies. This protein is predicted to contain 5 transmembrane domains localized in the mitochondrial inner membrane. Alternative splicing of this gene generates several transcript variants diverging in the 3' region including alternate poly A sites. In total, 2 different isoforms are encoded by these variants.1) Oquendo, C.E., (2004) J. Med. Genet. 41 (7), 540-544. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human COX15. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| COX15 antibody can be used for detection of COX15 by ELISA at 1:62500. COX15 antibody can be used for detection of COX15 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | COX15 |
|---|---|
| Alternative Names | COX15 CEMCOX2 |
| Molecular Weight(MW) | 44 kDa, 46 kDa |
Application
Antibody used in WB on Human HepG2 cells at 5.0 ug/ml.
Application
Antibody used in WB on Human fibroblast mitochondria at: 1:1000.
Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | COX15 antibody can be used for detection of COX15 by ELISA at 1:62500. COX15 antibody can be used for detection of COX15 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store COX15 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
