Application
COX7A2L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | COX7A2L Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cytochrome c oxidase subunit 7A-related protein, mitochondrial, COX7a-related protein, Cytochrome c oxidase subunit VIIa-related protein, EB1, COX7A2L, COX7AR, COX7RP |
| Product description | Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein similar to polypeptides 1 and 2 of subunit VIIa in the C-terminal region, and also highly similar to the mouse Sig81 protein sequence. This gene is expressed in all tissues, and upregulated in a breast cancer cell line after estrogen treatment. It is possible that this gene represents a regulatory subunit of COX and mediates the higher level of energy production in target cells by estrogen.1) Fornuskova, D., et al. Biochem. J. 428(3):363-374(2010) |
| Immunogen | This COX7A2L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the Central region of human COX7A2L. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COX7A2L |
|---|---|
| Alternative Names | Cytochrome c oxidase subunit 7A-related protein mitochondrial COX7a-related protein Cytochrome c oxidase subunit VIIa-related protein EB1 COX7A2L COX7AR COX7RP |
| Molecular Weight(MW) | 13 kDa |
Application
COX7A2L Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Application
Western Blot at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
