Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Product Name | CPSF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CPSF1 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly (A) polymerase and other factors to bring about cleavage and poly (A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.1) Beausoleil, S.A., (2004) Proc. Natl. Acad. Sci. U.S.A. 101 (33), 12130-12135. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CPSF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CPSF1 antibody can be used for detection of CPSF1 by ELISA at 1:62500. CPSF1 antibody can be used for detection of CPSF1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CPSF1 |
|---|---|
| Alternative Names | CPSF1 CPSF160 HSU37012 P/cl.18 |
| Molecular Weight(MW) | 161 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | CPSF1 antibody can be used for detection of CPSF1 by ELISA at 1:62500. CPSF1 antibody can be used for detection of CPSF1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CPSF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
