Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | CPSF7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CPSF7 is probable a component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3' processing. Binds to cleavage and polyadenylation RNA substrates.1) Olsen, J.V., et al. Cell 127(3):635-648(2006) |
| Immunogen | This CPSF7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 427-455 amino acids from the C-terminal region of human CPSF7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CPSF7 |
|---|---|
| Alternative Names | Cleavage and polyadenylation specificity factor subunit 7 Cleavage and polyadenylation specificity factor 59 kDa subunit CFIm59 CPSF 59 kDa subunit Pre-mRNA cleavage factor Im 59 kDa subunit CPSF7 |
| Molecular Weight(MW) | 52 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
