Application
Western blot analysis of CRB2 in rat brain tissue lysate with CRB2 antibody at (A) 0.5 and (B) 1 μg/mL| Product Name | CRB2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRB2 Antibody: Protein crumbs homolog 2, Crumbs-like protein 2 |
| Product description | CRB2 Antibody: CRB2 (Crumbs homolog 2), like its homologs CRB1 and CRB3, is similar to the Drosophila crumbs protein and is expressed in retina, brain and kidney. Along with other proteins, the Crumbs proteins form a complex that help set up cell polarity in developing neuroepithelial cells. At the onset of neural specification, embryonic stem cells (ESCs) upregulate CRB2, which then localizes apically in neural rosettes. Gain- and loss-of-function studies of CRB2 have shown that CRB2 is essential for the stabilization of other polarity proteins. Unlike CRB1, mutations in CRB2 do not appear to play a role in retinitis pigmentosa or in Leber congenital amaurosis.1) van den Hurk J, Rashbass P, Roepman R, et al. Characterization of the Crumbs homolog 2 (CRB2) gene and analysis of its role in retinits pigmentosa and Leber congenital amaurosis. Mol. Viso. 2005; 11:263-73 |
| Immunogen | Rabbit polyclonal CRB2 antibody was raised against a 17 amino acid peptide near the center of human CRB2. The immunogen is located within amino acids 460 - 510 of CRB2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CRB2 antibody can be used for detection of CRB2 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRB2 |
|---|---|
| Alternative Names | CRB2 Antibody: Protein crumbs homolog 2 Crumbs-like protein 2 |
| Molecular Weight(MW) | Predicted: 105, 129, and 141 kDa Observed: 103, 128 kDa |
| Tissue Specificity | At least three isoforms of CRB2 are known to exist; this antibody will detect all three. CRB2 antibody is predicted to not cross-react with other members of the CRB family. |
Application
Western blot analysis of CRB2 in rat brain tissue lysate with CRB2 antibody at (A) 0.5 and (B) 1 μg/mL| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | CRB2 antibody can be used for detection of CRB2 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CRB2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CRB2 Antibody is supplied in PBS containing 0.02% sodium azide. |
