Application
Antibody is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).Application
Western blot analysis of CKB using rabbit polyclonal CKB-C254 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.| Product Name | Creatine Kinase B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Creatine kinase B-type, B-CK, Creatine kinase B chain, CKB, CKBB |
| Product description | Creatine Kinase (CK) is a dimeric enzyme composed of either M- or B-type subunits. The resulting isoenzymes are expressed at varying levels in different tissues. CK-BB, a cytoplasmic predominantly found in brain tissues, participates in energy homeostasis, reversibly catalyzing the transfer of a phosphate group between ATP and target proteins such as a creatine phosphate. CK-BB exists in normally neglible amounts in the serum of adults, overexpression of CK-BB is associated with cancers of the breast, ovary, prostate, colon, and in small-cell lung cancer. Global assessment of changes in serum levels of CK-BB, CK-MB and CK-MM, are used as a marker for myocardial infarction. |
| Immunogen | This Creatine Kinase B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 240-270 amino acids from the C-terminal region of human Creatine Kinase B. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | CKB |
|---|---|
| Alternative Names | Creatine kinase B-type B-CK Creatine kinase B chain CKB CKBB |
| Molecular Weight(MW) | 43 kDa |
Application
Antibody is used in Western blot to detect CKB in Y79 cell lysate (Lane 1) and mouse colon tissue lysate (Lane 2).
Application
Western blot analysis of CKB using rabbit polyclonal CKB-C254 using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CKB gene.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
