WB
Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody at 1/2,000 dilution.Lysates/proteins at 15 µg/Lane.Exposure time: 2 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.ICC/IF
Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody at 1/2,000 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type.Cells were fixed and permeabilized. Then stained with the primary antibody (1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Product Name | Creatine kinase B type Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human CKB aa 1-381. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:2000 ICC/IF:1:2000 FC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CKB |
|---|---|
| Gene Synonyms | BCK B-CK CKBB CPK-B HEL-211 HEL-S-29 |
| Gene Full Name | creatine kinase B |
| Gene Summary | The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. A pseudogene of this gene has been characterized. [provided by RefSeq, Jul 2008] |
| Alternative Names | B CK antibody B-CK antibody BB-CK antibody BCK antibody Brain creatine kinase antibody Ckb antibody CKBB antibody Creatine kinase B antibody Creatine kinase B chain antibody Creatine kinase B type antibody B CK antibody B-CK antibody BB-CK antibody BCK antibody Brain creatine kinase antibody Ckb antibody CKBB antibody Creatine kinase B antibody Creatine kinase B chain antibody Creatine kinase B type antibody Creatine kinase B-type antibody Creatine Kinase BB Isoenzyme antibody Creatine kinase brain antibody Creatine kinase brain type antibody Creatine phosphokinase BB antibody Epididymis luminal protein 211 antibody Epididymis secretory protein Li 29 antibody HEL 211 antibody HEL S 29 antibody KCRB_HUMAN antibody |
| Molecular Weight(MW) | 43 kDa(Observed band size: 45 kDa) |
| Cellular Localization | Cytoplasm, cytosol, Mitochondrion, Cell membrane. |
WB
Western blot analysis of Creatine kinase B type on different lysates with Rabbit anti-Creatine kinase B type antibody at 1/2,000 dilution.Lysates/proteins at 15 µg/Lane.Exposure time: 2 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
ICC/IF
Immunocytochemistry analysis of SH-SY5Y cells labeling Creatine kinase B type with Rabbit anti-Creatine kinase B type antibody at 1/2,000 dilution.Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Creatine kinase B type antibody at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (594) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of SH-SY5Y cells labeling Creatine kinase B type.Cells were fixed and permeabilized. Then stained with the primary antibody (1/200) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/200dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).| Application Notes | WB:1:2000 ICC/IF:1:2000 FC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
