Application
Western blot analysis in mouse NIH-3T3,CHO cell line and mouse cerebellum tissue lysates (35ug/lane).Application
Immunohistochemical analysis of paraffin-embedded H. prostate section using CREBL1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Immunohistochemical analysis of paraffin-embedded H. kidney section using CREBL1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Western blot analysis of lysates from Hela, HepG2 cell line and human placenta tissue lysate (from left to right), using CREBL1Antibody at 1:1000 at each lane.| Product Name | CREBL1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CREBL1 is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha, the encoded protein binds to the ER stress response element, interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however, under ER stress, the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus.1) Guan, D., et al. J. Cell. Biochem. 108(4):825-831(2009) |
| Immunogen | This CREBL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 560-589 amino acids from the C-terminal region of human CREBL1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 | |
| Species Reactivity | HamsterHumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF6B |
|---|---|
| Alternative Names | Cyclic AMP-dependent transcription factor ATF-6 beta cAMP-dependent transcription factor ATF-6 beta Activating transcription factor 6 beta ATF6-beta Protein G13 cAMP response element-binding protein-related protein Creb-rp cAMP-responsive element-bi |
| Molecular Weight(MW) | 77 kDa |
Application
Western blot analysis in mouse NIH-3T3,CHO cell line and mouse cerebellum tissue lysates (35ug/lane).
Application
Immunohistochemical analysis of paraffin-embedded H. prostate section using CREBL1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded H. kidney section using CREBL1 Antibody . Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Western blot analysis of lysates from Hela, HepG2 cell line and human placenta tissue lysate (from left to right), using CREBL1Antibody at 1:1000 at each lane.| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
