The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. CREG1 antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro.The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.1) Sacher, M., (2005) Proc. Natl. Acad. Sci. U.S.A. 102 (51), 18326-18331.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CREG1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
CREG1 antibody can be used for detection of CREG1 by ELISA at 1:1562500. CREG1 antibody can be used for detection of CREG1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
CREG1 antibody can be used for detection of CREG1 by ELISA at 1:1562500. CREG1 antibody can be used for detection of CREG1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CREG1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.