Application
Western blot analysis of CRIM1 in Jurkat cell lysate with Crim1 antibody at 1 μg/mL.Application
Immunofluorescence of CRIM1 in Jurkat cells with CRIM1 antibody at 20 μg/mL.| Product Name | CRIM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CRIM1 Antibody: S52, CRIM-1, S52, UNQ1886/PRO4330, Cysteine-rich motor neuron 1 protein, Cysteine-rich repeat-containing protein S52 |
| Product description | CRIM1 Antibody: CRIM1 (cysteine-rich motor neuron 1), a glycosylated type I transmembrane protein, plays a role in tissue development i.e. capillary formation and maintenance during angiogenesis. It contains an N-terminal IGF-binding protein-like motif and six von Willebrand-like cysteine-rich repeats (CRRs) in its extracellular domain. CRIM1 interacts with BMP4 and BMP7 via the CRRs and functions as an antagonist. CRIM1 is developmentally expressed in a number of tissues including the pancreas, kidney, placenta, brain and blood vessels. CRIM1 may participate in CNS and placental development by interacting with growth factors involved in motor neuron differentiation and survival.1) Kolle G, Georgas K, Holmes GP, et al. CRIM1, a novel gene encoding a cysteine-rich repeat protein, is developmentally regulated and implicated in vertebrate CNS development and organogenesis. Mech. Dev. 2000; 90:181-93. |
| Immunogen | CRIM1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human Crim1. The immunogen is located within amino acids 910 - 960 of CRIM1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CRIM1 antibody can be used for detection of Crim1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRIM1 |
|---|---|
| Alternative Names | CRIM1 Antibody: S52 CRIM-1 S52 UNQ1886/PRO4330 Cysteine-rich motor neuron 1 protein Cysteine-rich repeat-containing protein S52 |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
| Tissue Specificity | CRIM1 antibody is human reactive. CRIM1 antibody is predicted to not cross-react with CRIM2. |
Application
Western blot analysis of CRIM1 in Jurkat cell lysate with Crim1 antibody at 1 μg/mL.
Application
Immunofluorescence of CRIM1 in Jurkat cells with CRIM1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | CRIM1 antibody can be used for detection of Crim1 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CRIM1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CRIM1 Antibody is supplied in PBS containing 0.02% sodium azide. |
