Application
Immunofluorescence of CRISP in human testis tissue with CRISP antibody at 20 μg/mL.Application
Immunohistochemistry of CRISP2 in human testis tissue with CRISP2 antibody at 10 μg/mL.Application
Western blot analysis of CRISP2 in human testis tissue lysate with CRISP2 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | CRISP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CRISP2 Antibody: The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.1) Cohen DJ, Busso D, Da Ros V, et al. Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction. Int. J. Dev. Biol.2008; 52:737-42. |
| Immunogen | CRISP2 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CRISP2. The immunogen is located within the first 50 amino acids of CRISP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CRISP2 antibody can be used for detection of CRISP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRISP2 |
|---|---|
| Alternative Names | CRISP2 Antibody: CT36 TPX1 TSP1 GAPDL5 CRISP-2 Cysteine-rich secretory protein 2 Testis-specific protein TPX-1 |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Entrez Gene | 7180 |
|---|---|
| Protein Accession | NP_001135880 |
Application
Immunofluorescence of CRISP in human testis tissue with CRISP antibody at 20 μg/mL.
Application
Immunohistochemistry of CRISP2 in human testis tissue with CRISP2 antibody at 10 μg/mL.
Application
Western blot analysis of CRISP2 in human testis tissue lysate with CRISP2 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | CRISP2 antibody can be used for detection of CRISP2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CRISP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CRISP2 Antibody is supplied in PBS containing 0.02% sodium azide. |
