Application
Western blot analysis of CROT Antibody in K562 cell line and mouse liver tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CROT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CROT encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.1) Li, Y.L., et al. FEBS J. 276(1):303-314(2009) |
| Immunogen | This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CROT |
|---|---|
| Alternative Names | Peroxisomal carnitine O-octanoyltransferase COT CROT COT |
| Molecular Weight(MW) | 70 kDa |
Application
Western blot analysis of CROT Antibody in K562 cell line and mouse liver tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
