Application
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.| Product Name | CRYGC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Crystallins are the dominant structural components of the vertebrate eye lens.Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.1) Leung, T., (er) Mol. Vis. 13, 1333-1338 (2007) . |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CRYGC. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CRYGC antibody can be used for detection of CRYGC by ELISA at 1:62500. CRYGC antibody can be used for detection of CRYGC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CRYGC |
|---|---|
| Alternative Names | CRYGC CCL CRYG3 CTRCT2 |
| Molecular Weight(MW) | 21 kDa |
Application
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.| Positive Control | 1) OVCAR-3 Cell Lysate |
|---|---|
| Application Notes | CRYGC antibody can be used for detection of CRYGC by ELISA at 1:62500. CRYGC antibody can be used for detection of CRYGC by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CRYGC antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
