Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CSPP1 Antibody in HL-60, Jurkat cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CSPP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CSPP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization.1) Asiedu, M., et al. Mol. Biol. Cell 20(5):1428-1440(2009) Patzke, S., et al. J. Cell. Physiol. 209(1):199-210(2006) Patzke, S., et al. Oncogene 24(7):1159-1173(2005) |
| Immunogen | This CSPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-333 amino acids from the N-terminal region of human CSPP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSPP1 |
|---|---|
| Alternative Names | Centrosome and spindle pole-associated protein 1 CSPP1 CSPP |
| Molecular Weight(MW) | 146 kDa |
Application
Flow cytometry analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CSPP1 Antibody in HL-60, Jurkat cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CSPP1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
