Application
This antibody stained HeLa cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.Application
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect Human CTACK by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human CTACK (38-274) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.| Product Name | CTACK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Produced from sera of rabbits immunized with highly pure recombinant Human CTACK. Human CTACK specific antibody was purified by affinity chromatography employing an immobilized Human CTACK matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Sandwich: To detect Human CTACK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human CTACK (38-274) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK. Western Blot: To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CCL27 |
|---|---|
| Alternative Names | ALP ILC CTAK CTACK PESKY ESKINE SCYA27 |
Application
This antibody stained HeLa cells. The primary antibody was incubated at 3.0 ug/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Application
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect Human CTACK by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human CTACK (38-274) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK.| Application Notes | ELISA: Sandwich: To detect Human CTACK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human CTACK (38-274) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTACK. Western Blot: To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The lyophilized antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-80˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
