Application
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | CtBP1 (phospho Ser422) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants.1) Schaeper U., Proc. Natl. Acad. Sci. U.S.A. 92:10467-10471(1995). |
| Immunogen | CtBP1 (phospho Ser422) antibody was raised against a peptide sequence around phosphorylation site of Serine 422 (A-P-S (p)-P-G) derived from Human CtBP1. |
| Modification | p-Ser422 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTBP1 |
|---|---|
| Alternative Names | CTBP C-terminal binding protein 1 EC 1.1.1 |
| Molecular Weight(MW) | 48 kDa |
| Tissue Specificity | The antibody detects endogenous levels of CtBP1 only when phosphorylated at serine 422. |
| Entrez Gene | 1487 |
|---|---|
| Protein Accession | NP_001319.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue, using CtBP1 (Phospho-Ser422) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Jurkat cells treated with TNF using CtBP1 (Phospho-Ser422) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
