Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.WB
C) Western blot analysis (left) and quantitative analysis (right) of CBS, CSE and MPST protein expression in lung cancer cell lines (A549 and 95D) and normal lung cell line (BEAS-2B).WB
B) Western blot analysis of CBS and CSE expressed in A549 cells after transfection.(H) Western blot analysis of CBS and CSE expressed in BEAS-2B cells after infection.WB
(C) Western blot analysis and qRT-PCR analysis of CBS, CSE and MPST expression after 200 μM CoCl2 treatment for 24 h.(F) Western blot and qRT-PCR analysis of H2S-producing enzymes and HIF-1α expression after transfection with pcDNA3.0/mut HIF-1α plasmid or empty vector control.(H) The expression levels of CBS, CSE, MPST and HIF-1α in protein and mRNA were detected using western blot and qRT-PCR analysis after transfection with si-HIF-1α or si-negative control (si-NC), respectively.WB
(C) Western blot analysis of CBS, CSE, and MPST protein expression levels.| Product Name | CTH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CTH is a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in its gene cause cystathioninuria.This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.1) Moore, L.E., (2007) Int. J. Cancer 120 (11), 2452-2458. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CTH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CTH antibody can be used for detection of CTH by ELISA at 1:312500. CTH antibody can be used for detection of CTH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTH |
|---|---|
| Alternative Names | CTH MGC9471 |
| Molecular Weight(MW) | 44 kDa |
Application
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
WB
C) Western blot analysis (left) and quantitative analysis (right) of CBS, CSE and MPST protein expression in lung cancer cell lines (A549 and 95D) and normal lung cell line (BEAS-2B).
WB
B) Western blot analysis of CBS and CSE expressed in A549 cells after transfection.(H) Western blot analysis of CBS and CSE expressed in BEAS-2B cells after infection.
WB
(C) Western blot analysis and qRT-PCR analysis of CBS, CSE and MPST expression after 200 μM CoCl2 treatment for 24 h.(F) Western blot and qRT-PCR analysis of H2S-producing enzymes and HIF-1α expression after transfection with pcDNA3.0/mut HIF-1α plasmid or empty vector control.(H) The expression levels of CBS, CSE, MPST and HIF-1α in protein and mRNA were detected using western blot and qRT-PCR analysis after transfection with si-HIF-1α or si-negative control (si-NC), respectively.
WB
(C) Western blot analysis of CBS, CSE, and MPST protein expression levels.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | CTH antibody can be used for detection of CTH by ELISA at 1:312500. CTH antibody can be used for detection of CTH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CTH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
