Application
Immunohistochemical analysis of paraffin - embedded human normal heart tissue showing cytoplasmic localization using cTnI antibody with DAB stainingApplication
Western blot analysis using cTnI monoclonal antibody against truncated cTnI recombinant protein.| Product Name | cTnI Antibody [3A10A12] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | cTnI has an apparent molecular weight of 22.5 kDa. cTnI is a candidate marker with acceptable sensitivity and specificity for AMI and other cardiac diseases. Troponin, a molecule that binds to the thin filament (actin) of striated muscle fibers, acts with intracellular calcium to control the interaction of the thin filament with the thick filament (myosin), thus regulating muscle contraction. Troponin I prevents muscle contraction in the absence of calcium, which has two skeletal muscle isoforms with considerable amino acid sequence homology. cTnI contains an additional N-terminal sequence and is highly specific for myocardium.1) Cummins B and Cummins P, J Mol Cell Cardiol, 1987, 19(10):999-1010. |
| Immunogen | Ni-NTA purified truncated recombinant cTnI expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:Dilution 1:500 - 1:2,000 IHC(P):Dilution 1:500 - 2,000 IHC(F):Dilution 1:500 - 2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TNNI3 |
|---|---|
| Alternative Names | TNNC1 Cardiac troponin ICMH7 RCM1 cTnI CMD2A TNNC1 CMD1FF |
Application
Immunohistochemical analysis of paraffin - embedded human normal heart tissue showing cytoplasmic localization using cTnI antibody with DAB staining
Application
Western blot analysis using cTnI monoclonal antibody against truncated cTnI recombinant protein.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | Western Blot:Dilution 1:500 - 1:2,000 IHC(P):Dilution 1:500 - 2,000 IHC(F):Dilution 1:500 - 2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
| Form | Liquid |
|---|---|
| Storage Instructions | cTnI monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
