Application
Immunofluorescence of CTRP1 in Human Kidney cells with CTRP1 antibody at 20 μg/mL.Application
Immunohistochemistry of CTRP1 in human kidney tissue with CTRP1 antibody at 10 μg/mL.Application
Western blot analysis of CTRP1 in human kidney cell lysate with CTRP1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CTRP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CTRP1 Antibody: Adipose tissue of an organism plays a major role in regulating physiologic and pathologic processes such as metabolism and immunity by producing and secreting a variety of bioactive molecules termed adipokines. One highly conserved family of adipokines is adiponectin/ACRP30 and its structural and functional paralogs, the C1q/tumor necrosis factor-alpha-related proteins (CTRPs) 1-7. Unlike adiponectin, which is expressed exclusively by differentiated adipocytes, the CTRPs are expressed in a wide variety of tissues. These proteins are thought to act mainly on liver and muscle tissue to control glucose and lipid metabolism. An analysis of the crystal structure of adiponectin revealed a structural and evolutionary link between TNF and C1q-containing proteins, suggesting that these proteins arose from a common ancestral innate immunity gene. In obese (ob/ob) mice, RT-PCR analysis showed that mCTRP1 transcripts are seen at substantially higher levels in adipose tissues compared to those of normal mice.1) Fantuzzi G. Adipose tissue, adipokines, and inflammation. J. Allergy Clin. Immunol. 2005; 115:911-9. |
| Immunogen | CTRP1 (NT) antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human CTRP1. The immunogen is located within amino acids 40 - 90 of CTRP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CTRP1 antibody can be used for the detection of CTRP1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C1QTNF1 |
|---|---|
| Alternative Names | CTRP1 Antibody: GIP CTRP1 ZSIG37 UNQ310/PRO353 Complement C1q tumor necrosis factor-related protein 1 G protein-coupled receptor-interacting protein GIP |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | These proteins are often highly modified post-translationally and migrate in SDS-PAGE at positions other than their predicted size. |
Application
Immunofluorescence of CTRP1 in Human Kidney cells with CTRP1 antibody at 20 μg/mL.
Application
Immunohistochemistry of CTRP1 in human kidney tissue with CTRP1 antibody at 10 μg/mL.
Application
Western blot analysis of CTRP1 in human kidney cell lysate with CTRP1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | CTRP1 antibody can be used for the detection of CTRP1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | CTRP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CTRP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
