Application
Western blot analysis of CTSK using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTSK gene (Lane 2).Application
Flow cytometric analysis of ZR-75-1 cells using(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human breast carcinoma with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CTSK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. This gene may be subject to RNA editing.1) Lendeckel,U., Neurochem. Int. 54 (7), 410-417 (2009) |
| Immunogen | This CTSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-237 amino acids from the Central region of human CTSK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTSK |
|---|---|
| Alternative Names | Cathepsin K Cathepsin O Cathepsin O2 Cathepsin X CTSK CTSO CTSO2 |
| Molecular Weight(MW) | 37 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of CTSK using rabbit polyclonal using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CTSK gene (Lane 2).
Application
Flow cytometric analysis of ZR-75-1 cells using(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human breast carcinoma with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
