WB
A549 cells were subjected to SDS PAGE followed by western blot with Cathepsin K antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Cathepsin K antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using Cathepsin K antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cathepsin K antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Cathepsin K Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | CTSK Fusion Protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:1000 IHC:1:100-1:400 ICC:1:200-1:800 IF-P:1:50-1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.55mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CTSK |
|---|---|
| Gene Synonyms | CTSO PKND PYCD CTS02 CTSO1 CTSO2 |
| Gene Full Name | cathepsin K |
| Gene Summary | The protein encoded by this gene is a lysosomal cysteine proteinase involved in bone remodeling and resorption. This protein, which is a member of the peptidase C1 protein family, is predominantly expressed in osteoclasts. However, the encoded protein is also expressed in a significant fraction of human breast cancers, where it could contribute to tumor invasiveness. Mutations in this gene are the cause of pycnodysostosis, an autosomal recessive disease characterized by osteosclerosis and short stature. [provided by RefSeq, Apr 2013] |
| Alternative Names | CTSK Cathepsin X catK CTSO CTSO2 |
| Molecular Weight(MW) | 39kDa(27 kDa, 38~46 kDa) |
| Cellular Localization | Cell membrane,Lysosome,Membrane,Secreted. |
WB
A549 cells were subjected to SDS PAGE followed by western blot with Cathepsin K antibody at dilution of 1:800 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using Cathepsin K antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed ROS1728 cells using Cathepsin K antibody at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Cathepsin K antibody at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:500-1:1000 IHC:1:100-1:400 ICC:1:200-1:800 IF-P:1:50-1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
