Application
Immunohistochemistry of CUEDC1 in human liver tissue with CUEDC1 antibody at 2 μg/mL.Application
Immunofluorescence of CUEDC1 in human liver tissue with CUEDC1 antibody at 20 μg/mL.Application
Western blot analysis of CUEDC1 in rat liver tissue lysate with CUEDC1 antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | CUEDC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUEDC1 Antibody: CUE domain-containing protein 1 |
| Product description | CUEDC1 Antibody: The CUE (coupling of ubiquitin conjugation to endoplasmic reticulum degradation) domain is an evolutionarily conserved, ~40 amino acid monoubiquitin-binding domain that mediates intramolecular monoubiquitylation. CUE domains are present in eukaryotic proteins that are involved in ubiquitination and protein trafficking pathways and may be required for ubiquitination of the proteins in which they are found. CUEDC1 (CUE domain-containing protein 1) is a 386 amino acid protein that contains one CUE domain, suggesting a possible role in protein trafficking and degradation pathways. Two isoforms of CUEDC1 exist due to alternative splicing events. Defects in the gene encoding CUEDC1 may be associated with early stage cervical cancer, implicating CUEDC1 as a potential tumor marker.1) Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J.2003; 22:1273-81. |
| Immunogen | CUEDC1 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human CUEDC1. The immunogen is located within amino acids 310 - 360 of CUEDC1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CUEDC1 antibody can be used for detection of CUEDC1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CUEDC1 |
|---|---|
| Alternative Names | CUEDC1 Antibody: CUE domain-containing protein 1 |
| Sequence Similarities | 2) Cat. No. 10-201 - Human Liver Tissue Slide |
| Tissue Specificity | CUEDC1 antibody will not cross-react with CUEDC2. |
Application
Immunohistochemistry of CUEDC1 in human liver tissue with CUEDC1 antibody at 2 μg/mL.
Application
Immunofluorescence of CUEDC1 in human liver tissue with CUEDC1 antibody at 20 μg/mL.
Application
Western blot analysis of CUEDC1 in rat liver tissue lysate with CUEDC1 antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | CUEDC1 antibody can be used for detection of CUEDC1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CUEDC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CUEDC1 Antibody is supplied in PBS containing 0.02% sodium azide. |
