Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation.Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Han, J. (2005) (er) Nucleic Acids Res. 33 (9), 2769-2780.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CUGBP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
CUGBP2 antibody can be used for detection of CUGBP2 by ELISA at 1:312500. CUGBP2 antibody can be used for detection of CUGBP2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Application
Antibody used in IHC on Human Heart at 4.0-8.0 ug/ml.
Application Notes
CUGBP2 antibody can be used for detection of CUGBP2 by ELISA at 1:312500. CUGBP2 antibody can be used for detection of CUGBP2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CUGBP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt