Application
Immunofluorescence of CXXC4 in human brain tissue with CXXC4 antibody at 20 μg/mL.Application
Immunohistochemistry of CXXC4 in human brain tissue with CXXC4 antibody at 10 μg/mL.Application
Western blot analysis of CXXC4 in human brain tissue lysate with CXXC4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | CXXC4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CXXC4 Antibody: CXXC4 is zinc finger protein that binds directly to the PDZ domain of DVL1, a protein involved in the Wnt signaling pathway, through its C-terminal region. CXXC4's interaction with DVL1 competes with DVL1-AXIN binding, although the affinity of DVL1 for CXXC4 is lower than that for AXIN. In mouse fibroblasts, CXXC4 suppressed Wnt3a induction of beta-catenin and inhibited Wnt3a-dependent TCF4 activation, suggesting that CXXC4 acts as a negative regulator of the Wnt signaling pathway and functions between DVL and beta-catenin. Recent reports suggest that decreased CXXC4 expression is associated with kidney cancer metastasis, cell proliferation, reduced apoptosis in response to cancer drugs, and upregulation of genes involved with proliferation, invasion and cell survival, suggesting that CXXC4 plays a critical role in tumor progression in kidney cancer.1) Hino S, Kishida S, Michuie T, et al. Inhibition of the Wnt signaling pathway by Idax, a novel Dvl-binding protein. Mol. Cell Biol.2001; 21:330-42. |
| Immunogen | CXXC4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CXXC4. The immunogen is located within amino acids 50 - 100 of CXXC4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CXXC4 antibody can be used for detection of CXXC4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXXC4 |
|---|---|
| Alternative Names | CXXC4 Antibody: IDAX IDAX CXXC-type zinc finger protein 4 Inhibition of the Dvl and axin complex protein |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunofluorescence of CXXC4 in human brain tissue with CXXC4 antibody at 20 μg/mL.
Application
Immunohistochemistry of CXXC4 in human brain tissue with CXXC4 antibody at 10 μg/mL.
Application
Western blot analysis of CXXC4 in human brain tissue lysate with CXXC4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Application Notes | CXXC4 antibody can be used for detection of CXXC4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
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| Form | Liquid |
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| Storage Instructions | CXXC4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CXXC4 Antibody is supplied in PBS containing 0.02% sodium azide. |
