Application
Immunohistochemistry of CXXC5 in human brain tissue with CXXC5 antibody at 2.5 μg/mL.Application
Western blot analysis of CXXC5 in human brain tissue lysate with CXXC5 antibody at 1 μg/mL.Application
Immunofluorescence of CXXC5 in Human Brain tissue with CXXC5 antibody at 20 μg/mL.| Product Name | CXXC5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CXXC5 Antibody: CXXC5 is zinc finger protein closely related to the DVL1-binding protein CXXC4 (also known as Idax), and like CXXC4 is thought to modulate the Wnt signaling pathway during development. CXXC5 is upregulated in neural stem cells upon BMP4 stimulation, leading to decreased levels of the Wnt-signaling target AXIN2 and facilitated the response of the stem cells to Wnt3a. Recent reports suggest CXXC5 transcription is activated by the Wilms tumor 1 (WT1) transcription factor, and together are involved in the WNT/beta-catenin pathway and play a role in embryonic kidney development.1) Andersson T, Sodersten E, Duckworth JK, et al. CXXC5 is a novel BMP4-regulated modulator of Wnt signaling in neural stem cells. J. Biol. Chem.2009; 284:3672-81. |
| Immunogen | CXXC5 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human CXXC5. The immunogen is located within amino acids 30 - 80 of CXXC5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CXXC5 antibody can be used for detection of CXXC5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXXC5 |
|---|---|
| Alternative Names | CXXC5 Antibody: CF5 WID RINF HSPC195 CXXC-type zinc finger protein 5 Putative MAPK-activating protein PM08 CF5 |
| Molecular Weight(MW) | Predicted: 25, 35 kDa Observed: 25, 35 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least two isoforms of CXXC5 are known to exist. CXXC5 antibody is predicted to not cross-react with other CXXC protein family members. |
Application
Immunohistochemistry of CXXC5 in human brain tissue with CXXC5 antibody at 2.5 μg/mL.
Application
Western blot analysis of CXXC5 in human brain tissue lysate with CXXC5 antibody at 1 μg/mL.
Application
Immunofluorescence of CXXC5 in Human Brain tissue with CXXC5 antibody at 20 μg/mL.| Application Notes | CXXC5 antibody can be used for detection of CXXC5 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CXXC5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CXXC5 Antibody is supplied in PBS containing 0.02% sodium azide. |
