Application
Western blot analysis of extracts from MCF7 tissue using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | Cyclin D1 (phospho Thr286) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and auto-ubiquitination of STUB1, TRAF6 and TRIM63/MURF1. Ubiquitinates STUB1-associated HSP90AB1 in vitro. Lacks inherent specificity for any particular lysine residue of ubiquitin. Essential for viral activation of IRF3. Mediates polyubiquitination of CYP3A4.1) Liou YC, et al. (2002) Proc Natl Acad Sci U S A 99, 1335-40 |
| Immunogen | Cyclin D1 (phospho Thr286) antibody was raised against a peptide sequence around phosphorylation site of threonine 286 (A-C-T (p)-P-T) derived from Human Cyclin D1. |
| Modification | p-Thr286 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCND1 |
|---|---|
| Alternative Names | BCL-1 oncogene CYL-1 PRAD1 PRAD1 |
| Molecular Weight(MW) | 33 kDa |
| Tissue Specificity | The antibody detects endogenous level of Cyclin D1 only when phosphorylated at threonine 286. |
| Entrez Gene | 595 |
|---|---|
| Protein Accession | NP_444284.1 |
Application
Western blot analysis of extracts from MCF7 tissue using Cyclin D1 (Phospho-Thr286) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
