Application
Antibody used in WB on Human lung microsome at: 1:1000 (Lane 1: Human lung microsome lysate, Lane 2-5: 150 ug mouse lung microsome lysate).Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Product Name | CYP1B1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP1B1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP1B1 localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid.This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The enzyme encoded by this gene localizes to the endoplasmic reticulum and metabolizes procarcinogens such as polycyclic aromatic hydrocarbons and 17beta-estradiol. Mutations in this gene have been associated with primary congenital glaucoma; therefore it is thought that the enzyme also metabolizes a signaling molecule involved in eye development, possibly a steroid. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Ikeda, S., (2008) Am. J. Gastroenterol. 103 (6), 1476-1487. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CYP1B1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CYP1B1 antibody can be used for detection of CYP1B1 by ELISA at 1:62500. CYP1B1 antibody can be used for detection of CYP1B1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP1B1 |
|---|---|
| Alternative Names | CYP1B1 CP1B GLC3A CYPIB1 P4501B1 |
| Molecular Weight(MW) | 61 kDa |
Application
Antibody used in WB on Human lung microsome at: 1:1000 (Lane 1: Human lung microsome lysate, Lane 2-5: 150 ug mouse lung microsome lysate).
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Application Notes | CYP1B1 antibody can be used for detection of CYP1B1 by ELISA at 1:62500. CYP1B1 antibody can be used for detection of CYP1B1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CYP1B1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
