Application
Western blot analysis of CYP2F1 Antibody in A2058 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP2F1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CYP2F1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | CYP2F1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin.1) Tournel,G., Xenobiotica 37 (12), 1433-1438 (2007) |
| Immunogen | This CYP2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-424 amino acids from the C-terminal region of human CYP2F1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | CYP2F1 |
|---|---|
| Alternative Names | Cytochrome P450 2F1 CYPIIF1 CYP2F1 |
| Molecular Weight(MW) | 56 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of CYP2F1 Antibody in A2058 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP2F1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
