Application
Confocal immunofluorescent analysis of CYP2S1 Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
CYP2S1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysate from BEAS 2B cell line, using CYP2S1 Antibody at 1:1000.Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Product Name | CYP2S1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. In rodents, the homologous protein has been shown to metabolize certain carcinogens; however, the specific function of the human protein has not been determined.1) Bui, P.H., et al. Mol. Pharmacol. 76(5):1031-1043(2009) Saito, A., et al. J. Hum. Genet. 54(6):317-323(2009) Deb, S., et al. Expert Opin Drug Metab Toxicol 5(4):367-380(2009) Jang, Y.J., et al. Ther Drug Monit 29(3):292-298(2007) Hanzawa, Y., et al. Drug Metab. Pharmacokinet. 22(2):136-140(2007) |
| Immunogen | This CYP2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 399-428 amino acids from the C-terminal region of human CYP2S1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CYP2S1 |
|---|---|
| Alternative Names | Cytochrome P450 2S1 CYPIIS1 CYP2S1 |
| Molecular Weight(MW) | 56 kDa |
Application
Confocal immunofluorescent analysis of CYP2S1 Antibody with HepG2 cell followed by Alexa Fluor488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
CYP2S1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysate from BEAS 2B cell line, using CYP2S1 Antibody at 1:1000.
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
